Detection of Apoptosis Initiated in Treated HepG2 Cells with t-BHP: The Role of Phytochemicals to Reduce Toxicity and Stop Apoptosis

Apoptosis or programmed cell death is a standard physiological mechanism. It essential to control the number of cells, balance division and death, regulate immune system, eliminate pathogen-infected cells. entailed different investigation determine related biochemical reactions such as activated caspase, Reactive Oxygen Species (ROS), Lipid Peroxidation (LPO), Evaluation Glutathione Content (GSH) by using techniques. HepG2 cells were exposed +/- 0.4 0.8 mM t-BHP for specific times induce toxicity apoptosis detection. We aim investigate mechanism in treated with under consideration conditions cytoprotection assay. Results showed no strong evidence apoptosis, although caspase-3 activity increased significantly (p ≤ 0.05) HpG2 at 150 minutes. The weak proof may attribute participation Calpain through cross-talk blocking caspase- activation. Similarly, we obtained significant ROS lipid peroxidation increases 0.05 0.01 respectively) Moreover, reported (non-significant) decline GSH amounts. Treatment Q I3C used study prevented activation identified western blot.